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- MedChemExpress(MCE)
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- 2025年12月05日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C, protect from light
- 库存：
货期：1-2天
- 供应商：
MedChemExpress LLC
- 规格：
100 μg
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Flutax-2 (5/6-mixture)
MCE 国际站：Flutax-2 (5/6-mixture)
产品活性：Flutax-2 (5/6-mixture) 是紫杉醇的活性荧光衍生物。Flutax-2 (5/6-mixture) 与聚合的 α,β 微管蛋白二聚体结合。激发/发射波长：496/524 nm。紫杉醇是一种由红豆杉属植物产生的二萜次生代谢物，可用于多种癌症的研究。
研究领域：Others | Cell Cycle/DNA Damage | Cytoskeleton
作用靶点：Fluorescent Dye | Microtubule/Tubulin
In Vitro: Preparation of Flutax-2 working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of Flutax-2 in 1 mL of DMSO.
Note: It is recommended to store the stock solution at -20℃or -80℃away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Flutax-2 working solution
Dilute the stock solution in sacetate buffer to obtain 100 nM-1 μM of Flutax-2 working solution.
Note: Please adjust the concentration of Flutax-2 working solution according to the actual situation.
Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Flutax-2 working solution, and then incubate at room temperature for 10-30 minutes.
2.3 Centrifuge at 400 g at 4℃for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
Storage
-20℃, 1 year
Protect from light
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文献和实验Biotin labeling and hybridization of probe to chromosomes
hybridization mixture: deionized formamide 10 µl 20 X SSC 2 µg
之mycoplasma (e.g. M. hyorhinis)。不需培养mycoplasma作为正反应对照组，避免可能之污染。 缺点︰PCR反应很灵敏，易有伪阳性结果。此方法尚在评估中，故结果仅作为参考和内部品管之一部份。 材料与设备： ATCC mycoplasma detection kit:可作50～100 reactions. 提供1st stage primer mixture与2nd stage primer mixture positive control DNA (A. laidlawii
In Situ Hybridization Using Digoxigenin Labeled Probes
siliconized slide place two drops of 45% acetic acid and a clean siliconized 18 mm square coverslip. On the coverslip place a small drop (2-3 mm) of fixative( 1/2/3 mixture of lactic acid/water/glacial acetic acid) 3) In the first drop of 45% acetic
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