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抗酪氨酸羟化酶抗体 预稀释克隆LNC1 小鼠源 免疫组化石蜡

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  • 询价
  • IHCR1005-6
  • 2025年07月14日
  • Immunohistochemistry (Paraffin)
  • Mouse
  • Chicken;Human;Mouse;Monkey
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      Immunohistochemistry (Paraffin)

    • 宿主

      Mouse

    • 抗原来源

      0

    • 适应物种

      Chicken;Human;Mouse;Monkey

    • 库存

      大量

    • 是否单克隆

      2

    • 规格

      6 mL

    Species Reactivity Key Applications Host Format Antibody Type
    Ch, H, M, Mk, R, Fg, Lz, Vo, Zebrafish IH(P) Mouse Purified Polyclonal Antibody
    Description:
    Anti-Tyrosine Hydroxylase, prediluted, clone LNC1
    Background Information:
    Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
    Clone:
    LNC1
    Specificity:
    Tyrosine Hydroxylase. Recognizes a protein of approximately 59-63 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase, phenethanolamine-N-methyl transferase (PNMT).
    Key Applications:
    Immunohistochemistry (Paraffin)
    Application Notes:
    Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

    Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

    Incubation: 30 minutes with IHC Select® Detection Kits.

    Tyrosine Hydroxylase has been prediluted for use as the primary antibody with Chemicon's IHC Select® Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier's IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR1005-6.
    Species Reactivity:
    • Chicken
    • Human
    • Mouse
    • Monkey
    • Rat
    • Frog
    • Lizard
    • Vole
    • Zebrafish
    Isotype:
    IgG1κ
    Immunogen:
    Tyrosine Hydroxylase purified from PC12 cells.
    Epitope:
    Recognizes an epitope on the outside of the regulatory N-terminus of tyrosine hydroxylase.
    Presentation:
    Liquid diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% ProClin 300 as preservative.
    Storage Conditions:
    Maintain at 2-8°C. Refer to vial for expiration dating.
    Gene Symbol:
    • TH
    • TYH
    • EC 1.14.16.2
    Antibody Category:
    Neuroscience
    Antibody Sub-Category:
    Neuronal & Glial Markers
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    Purified
    Host:
    Mouse
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    Tyrosine Hydroxylase purified from PC12 cells. 详细描述见链接:http://www.millipore.com/catalogue/item/IHCR1005-6

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    图标文献和实验
    相关实验
    • 免疫组化常见十大问题与解析

      Polymer二抗进行了反应的放大,该方法敏感性较高,可缩短抗体孵育时间或降低抗体浓度。 (2)内源性过氧化物酶残余:适度延长内源性过氧化物酶阻断剂阻断时间。 (3)一抗导致的非特异性染色:多克隆抗体易于产生非特异性染色,结合抗体官网信息合理选择多克隆抗体或单克隆抗体。 (4)一抗种属来源与组织样本来源相同:如使用鼠源一抗检测小鼠组织,尽量选择种属来源与样本种属不同的一抗。 (5)切片或细胞过于干燥:实验过程中,需保持样本的湿润。 Q5:阳性染色细胞定位不正确,如何修正? (1)抗原修复条件不合理:尝试改变

    • 进阶:6 年免疫组化经验总结

      地命中目标一样。另一方面,即使是同一个抗原决定簇,在机体内也可以由好几种克隆来产生抗体,形成好几种单克隆抗体混杂物,称为多克隆抗体。在抗原抗体反应中,一般单克隆抗体特异性强,但亲和力相对小,检测抗原灵敏度相对就低;而多克隆抗体特异性稍弱,但抗体的亲和力强,灵敏度高,但易出现非特异性染色(可以通过封闭等避免)。   2、应用范围的选择。有的一抗只能用于 WB ( Western blotting)或免疫组化、免疫荧光、免疫沉淀等;甚至表明石蜡切片或冰冻切片。   3、种属反应性的选择。这一点很重

    • 6年免疫组化经验总结

      抗体能目标明确地与单一的特异抗原决定簇结合,就像导弹精确地命中目标一样。另一方面,即使是同一个抗原决定簇,在机体内也可以由好几种克隆来产生抗体,形成好几种单克隆抗体混杂物,称为多克隆抗体。在抗原抗体反应中,一般单克隆抗体特异性强,但亲和力相对小,检测抗原灵敏度相对就低;而多克隆抗体特异性稍弱,但抗体的亲和力强,灵敏度高,但易出现非特异性染色(可以通过封闭等避免)。 2.应用范围的选择。有的一抗只能用于 WB ( Western blotting)或免疫组化、免疫荧光、免疫沉淀等;甚至表明石蜡切片

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