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文献和实验免疫组化技术-Immunohistochemical Protocols
Co, Phillisburg, NJ #57-50-1), paraformaldehyde (Polysciences, Warrington, PA; #0380), bovine serum albumin (ICN Biomedicals, Aurora, OH; #160069), Triton X-100
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
. Repeat Steps 25.iii-25.iv. vii. Resuspend the beads in 1 mL of 0.5% BSA in PBS. Rotate end-over-end overnight at 4°C. 26. Add 100 µL of the blocked magnetic bead solution to each immunoprecipitate sample
-react or otherwise disrupt the specific probing interaction being studied. Determination of an effective blocking buffer must be made empirically. Often, bovine serum albumin (BSA) is used as a starting point for many membrane-probing reactions
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