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- 2025年07月15日
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上海玉博生物科技有限公司
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文献和实验for producing multipotent Kdr?expressing mesoderm progenitor cells in serum?free conditions, and for functional genomics screening using these cells. Kdr?positive cells are able to differentiate into a wide array of mesodermal derivatives, including vascular
Preparation of cytoplasmic extracts for the application in a cell-free system
cells in a 160 cm2 flask (25 ml medium) up to about 80% cell density. Remove the RPMI medium and detach cells by incubating with 10 ml trypsin/EDTA (Sigma) for 5 min at 37°C For the inactivation of trypsin, add 10 ml of Bovine Calf Serum (BCS
Preparation of cytoplasmic extracts for the application in a cell-free system
cells in a 160 cm2 flask (25 ml medium) up to about 80% cell density. Remove the RPMI medium and detach cells by incubating with 10 ml trypsin/EDTA (Sigma) for 5 min at 37°C For the inactivation of trypsin, add 10 ml of Bovine Calf Serum (BCS
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