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文献和实验Cyanine Dye Purification Protocol
Low salt buffer (LSB): 25mM triethylammonium acetate, pH 8.0 10% acetonitrile (this is 40-fold dilution of HSB + 10% acetonitrile) HSB, 800 mL To 500mL ddH2 O , add approx. 25-30 mL glacial acetic acid
. For these challenging antigen-down assays CANDOR has developed PlateBlock™, which was optimized to prevent the exchange reactions in the most comprehensive way by forming a very resilient blocking layer on plastic surfaces (Fig. 2). In combination with LowCross
of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
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