santa cruz特约代理商|sc2003 Protein

lution of GST-Fusion Protein from Glutathione Agarose

  lution of GST-Fusion Protein from Glutathione Agarose 1. Wash the GST-fusion protein glutathione-agarose complex once with 1% Triton X-100 in PBS. 2. Resuspend the complex in 1% Triton X-100 in PBS. 3. Prepare a small column

2003年国际主要分子生物学数据库

.nih.gov/SNP/ Single nucleotide polymorphisms 175.  FLAGdb/FST http://genoplante-info.infobiogen.fr/ Arabidopsis thaliana T-DNA transformants 176.  GRAP Mutant Databases http://tinyGRAP.uit.no/GRAP/ Mutants of family A G-Protein Coupled Receptors

Chromatin Immunoprecipitation (ChIP) of Protein Complexes: Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells

, NTPs, buffer, Mg++ ) Phenol/chloroform/isoamyl alcohol Protein A/G agarose beads (50%), pre-swollen and blocked (Santa Cruz, sc-2003) Proteinase K (20 mg/ml; store at -20°C) Random-primed DNA synthesis kit RIPA buffer