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上海玉博生物科技有限公司
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文献和实验-acetate, pH 7.5 0.3 M NaCl 0.5% IGEPAL CA-630 0.1% sodium dodecyl sulfate (SDS)5 0.02% NaN3 Store at 25 C indefinitely Protein A-Sepharose CL-4-B Suspension6 Protein A-Sepharose beads (Pharmacia or Sigma) are swelled in PBS or IMP buffer on a tube
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
dodecyl sulfate (SDS)5 0.02% NaN3 Store at 25 C indefinitely Protein A-Sepharose CL-4-B Suspension 6 Protein A-Sepharose beads (Pharmacia or Sigma) are swelled in PBS or IMP buffer on a tube turner at room temperature. Beads are then washed
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
immediately before use by diluting 1:50 into 1 x PBS. Paraformaldehyde Fixative (4%):4 g solid paraformaldehyde2 mM MgC121.25 mM EGTA (0.25 ml of a .5M EGTA stock pH 8.0) in 100 ml PBS, pH 7.2-7.4Heat H20 to 60°C. Add paraformaldehyde. Add NaOH
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