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文献和实验Porcine brain tubulin preparation
1. 9. Transfer the phosphocellulose resin to the 4 L beaker, add 2 L of 10 x column buffer. Mix gently for 10-15 min, allow the resin to settle, aspirate excess column buffer off, and vacuum filter. 10. Transfer the resin to the 4 L beaker, add
x Column Buffer ~10 L 1x column buffer 10 M NaOH 3 fresh ( Equipment: Waring Blender Temperature controlled ultracentrifuge (Beckman L7-55) with the equivalent of a Beckman 50.2Ti rotor At least 24 31.5 ml thick-walled polycarbonate ultracentrifuge tubes
Mix, settle, aspirate, and vacuum filter as in step 1. Transfer the phosphocellulose resin to the 4 L beaker, add 2 L of 10 x column buffer. Mix gently for 10-15 min, allow the resin to settle, aspirate excess column buffer
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