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上海玉博生物科技有限公司
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文献和实验Steady State ATPase Assays Coupled Enzyme System
, in Tris-HCl. Avoid using phosphate buffers for ATPase assays. 2. The coupled enzyme ATPase assay is based on the conversion of phosphoenolpyruvate (PEP) to pyruvate by pyruvate kinase (PK) coupled to the conversion
transferase (CAT), β-galactosidase, and luciferase. Sensitive assays are available for each of these proteins that facilitate detection and quantitation of transgene expression. The use of these reporter enzymes allows a more rapid and sensitive method
Capillary Electrophoretic Enzyme Assays
In the past years, capillary electrophoresis has become a frequently used technique for enzyme assays due to the high separation efficiency and versatility as well as small sample size and low consumption of chemicals
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