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文献和实验Characterizing Bile Acid and Lipid Metabolism in the Liver and Gastrointestinal Tract of Mice
Ethanol (HPLC grade) 1 mg/ml deuterated glycol‐cholic acid (glycol‐CA; Point‐Clare) Ultrapure H 2 O
2 O to 1 L 1 M DTT (Dithiothreitol, Cleland's reagent): 1.54 g DTT (Calbiochem 233155) ddH2 O to 10 ml (aliquot and store at -20℃). DNase-free RNase A 20 mg/ml RNase A in 1 mM NaOAc, pH 4.5. 200 mg RNase A (Sigma R-5500) 3.3 μl 3 M NaOAc, pH
Raft culture media (aka Green’s media)
AcCOOH + 9.9ml H2 O) I had to add more glacial acetic acid to make it dissolve. Store working aliquots at 4C. Hydrocortisone (Sigma H0135), MW=362, final concentration=400ng/ml, working stock=1mg/ml, Dilution factor=2500.
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