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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1
- 供应商:
上海玉博生物科技有限公司
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文献和实验of a 10% gel 4 ml H2O 3.3ml 30% acrylamide/bisacrylamide (29:1 mix) 2.5 ml1.5M Tris(PH 8.8) 10% SDS 100 µl 10% ammonium persulfate 100 µl TEMED 8 µl 2. Stacking gel: 4 ml 2.7ml water 1 ml 0.67ml 30% acrylamide/bisacrylamide (29:1 mix) 0.ml 1.0M
by carefully adding 1 x SDS running buffer to unit. Avoid pouring the buffer directly onto the wells.7. Run gels 200V for 30-45 min until dye front is at the bottom of gel. (Large BioRad gels -15 X 15cm - are run at 35mA per gel).8. See below for Western
by carefully adding 1 x SDS running buffer to unit. Avoid pouring the buffer directly onto the wells.7. Run gels 200V for 30-45 min until dye front is at the bottom of gel. (Large BioRad gels -15 X 15cm - are run at 35mA per gel).8. See below for Western
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