eylabs特约代理商|H-1301-1 HRP CONJU

免疫沉淀-蛋白印迹法

(HRP)-conjugated IgG; Rabbit Anti-Mouse horseradish peroxidase were from Perbio Science UK Ltd (Cheshire, UK).Heparin (sodium salt Grade A), glycerol, methanol, Bromophenol Blue (sultone form), 2-mercaptoethanol, glycine, leupeptin, aprotenin, NaCl

Stripping Western Blots

. 4mls/10cm plate), add fresh trypsin/ EDTA (e.g. 1ml/10cm plate), and incubate at 37oC as appropriate to loosen cells (e.g. 1-2min for NIH3T3, 2-5min for MDCK). 3. Add 4 volumes of soybean trypsin inhibitor (1mg/ml in serum-free medium) e.g. 4mls

v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平

that its sensitivity is improved in 1% milk without lossof specificity. 6. The luminescence emitted by the ECL/horseradish peroxidase(HRP)-conjugated secondary antibodies decreases withtime, so it is best to expose the membranes to film immediatelyafter addition