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上海玉博生物科技有限公司
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文献和实验IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBRYOS
on a Millipore device with a nitex filter; rinse well with 0.1% Triton X-100 from a squirt bottle. Transfer embryos to 3:1 heptane:fix with a paint brush and fix for 20-40 min. Fix in a glass scintillation vial using 6.1ml HEPES Fix Mix + 0.75ml 37
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBR
. Transfer embryos to 3:1 heptane:fix with a paint brush and fix for 20-40 min. Fix in a glass scintillation vial using 6.1ml HEPES Fix Mix + 0.75ml 37 formaldehyde + heptane to more-or-less fill the vial. For very small collections, stab vials
the volume of media required to give a final density of 3.0 x 107 cells/ml. This density is very important, do not deviate from it. 8. Pellet cells by centrifuging @ 1000 rpm for 7 minutes. 9. Aspirate off supernatant and resuspend the pellet
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