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文献和实验Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
phase (upper layer). 6. Place the aqueous phase into two 0.5" x 2" polyallomer tubes (for SW50.1 rotor) and add NaCl to a final concentration of 0.1 M NaCl. 7. Add 3 volumes of 100% Ethanol, cover with Parafilm, and mix carefully
Sample Preparation for Microinjection
of microinjected cells. Between 500 and 1000 labeled cells are sufficient to analyze labeled proteins by gel electrophoresis. Cultivation and Microinjection Preparation [3] Plate 250 cells in 5 µl droplets in the center of a glass coverslip (10 x
Sample Preparation for Microinjection
these substances are typically mixed with dyes or labeled with fluorescent markers such as flourescein or rhodamine. After the capillary has pierced the cell, a certain amount of transfer substance (approximately 10% of the cell volume) is transferred
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