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文献和实验(Sigma G-4505) 50 ml 1 M Tris-HCl, pH 7.6 40 ml 0.5 M EDTA, pH 8.0 ddH2 O to 1 liter GET/lysozyme solution : (50 mM glucose, 25 mM Tris-HCl, pH 8.0, and 10 mM EDTA, pH 8.0 in double distilled water) 0.9 g d-glucose 2.5 ml 1 M Tris-HCl, pH
QUALITATIVE ANALYSIS OF DNA FRAGMENTATION BY AGAROSE GEL ELECTROPHORESIS
min. Let air dry the tubes in upright position for at least 3 hr before proceeding. 16. Dissolve DNA by adding to each tube 20-50 m l of TE solution and place the tubes at 37°C for 1-3 days. The redissolution of DNA may be a crical step, in fact
Alkaline Lysis and CsCl Purification of BAC DNA for Transgenic Production.
BAC DNA is the lower band). Remove band in the smallest possible volume (should be 21. Extract EtBr with TE pH 7.5/100mM NaCl-saturated butanol (1:1 volume ratio), >5 times, or at least 1 extraction past step where all orange color is removed
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