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文献和实验Dual- and Triple-Color Fluorospot
3, and Cy5. 3. Fluorescence Enhancer (Mabtech) is used to improve the fluorescent signal. 实验步骤 The protocol describes double staining for the detection of human IFN-γ and IL-2. PVDF plates are coated with mAbs to IFN-γ and IL-2
Immunohistochemistry using Anti-Ganglioside Antibodies
')2 or FITC-conjugated goat anti-mouse IgG F (ab')2 diluted at 1:1000 each with 1% BSA in PBS for 1 hr. at room temperature. 7. Wash with 1% BSA in PBS 5 times. 8. Apply drops of fluorescence mounting medium on section and mount micro cover glass
Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry
Human hematopoietic stem cells (HSCs) and progenitors can be isolated by enriching for a rare cell population with a combination of monoclonal antibodies (MAbs). Such an isolation scheme involves multi-step procedures including
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