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文献和实验Methanol Fixation for Immunofluorescence
. We recommend that you try both types of fixation for your particular antibody/fusion protein when working out your initial immunofluorescence conditions. MeS Buffer 100 mM MeS, pH 6.9 1 mM EGTA 1 mM MgCl2 Store at 4°C Methanol Fix (100
, 100 ml, warm. 7. Fixation boxes/dished. Procedure 1. Rinse coverslip 2x with warm phosphate buffered saline. 2. Fix 1 min in buffer 3. Agitate periodically. 3. Rinse 2x with cytoskeleton buffer. 4. Fix 15 min
Pouring Linear and Buffer-Gradient Sequencing Gels
to be read from a single run) up to 100 cm in length can be poured, as can wider gels (to enable more clones to be loaded onto a single gel). However, these larger gels are more difficult to handle after electrophoresis, i.e., during fixation, drying
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