Interchim特约代理商|CD3820 INTERCHI
M SPIN-25 CA FILTER .2MU 25ML RECEIVER TUBE+CAP 01 x 50 U.- ￥3000
- Corning
- 431082
- 2025年07月08日
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文献和实验He Tube Mating with MSCV recipient
. 3. Set up Dilutions: Set up dilutions as shown below, in 5 ml LB+0.2% rhamnose. Use 50 ml Falcons for this as they have good aeration and you get much better growth. Place these dilutions at 30℃ shaking for 2 hours. pMSCV: 25 μl 5ml 50 μl 5ml
Filter Binding Assay for EBNA-1
Wash one 0.45um Millipore nitrocellulose filter (Cat# HAWP01300) in 1ml 0.4M KOH for 1 min; wash in 3ml dH2 O for >1min. Assemble filter apparatus: white plastic bottom support/funnel, nitrocellulose filter, white plastic top with syringe adapter
Fastfilter Plasmid Midi Kit Spin Protocol
plasmid purity. Prolonged incubation may lead to nicking of plasmid DNA. (Store Solution II tightly capped when not in use.) 5. Add 1.25 ml ice-cold Buffer N3, cover, and gently mix by inverting tube several times until a flocculent white precipitate
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