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文献和实验SOUTHERN BLOTTING OF GENOMIC DNA
blotting membrane,wicks,etc.,since oils on skin will inhibit transfer.WASH OFF powder from gloves before use. • Soak gel 20-40 minutes in denaturation solution (0.4 M NaOH,0.8 M NaCI) • Set up capillary blot apparatus (this is one of several transfer
2 Dimensional Gel Electrophoretic Analysis for Chicken Egg
Biotech) 13. The glass staining basin 14. The fixation solution: 100 ml Ethanol 25 ml Acetic acid glacial � Make up to 250 ml with distilled H2O 15. The sensitizing solution: 75 ml Ethanol 1.25 ml Glutardialdehyde (25% w/v) 10 ml
2 Dimensional Gel Electrophoretic Analysis for Chicken Egg
wicks soaked with buffer solution on both the positive and negative electrodes. 15. Seal the liquid surface with a layer of mineral oil to minimize evaporation. 16. Cover the IEF tray with the lid. 17. Run IEF: Step 1: 500 volts, 1 hour
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