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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
, dilute 1000x Me-JA 98% solution, 5ul/1ml EtOH, 100ul/9.9ml Inc. Firmenich Geneva buffer/media, dile 10 or 100x. Protein synthesis inhibitors cycloheximide: [active] 20mM, stock 10mM, MW 281, stock = Boe 103675 2.8mg/ml EtOH, dilute 1000x
Coomassie Brilliant Blue G-250 in 50 ml 95% ethanol,add 100 ml 85% (w/v)phosphoric acid.Dilute to 1 liter when the dye has completely dissolved,and filter through Whatman #1 paper just before use.2.(Optional)1 M NaOH (to be used if samples are not readily
3MM paper) and make up to 180ml with H2O. 5) Add 20ml fresh 10 x TBE buffer. 6) For a gel of ~100ml, use 200ul TEMED and 200ul fresh 25% AMPS solution to initiate polymerisation. N.B.: For the above quantities of TEMED and AMPS, you MUST chill
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