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文献和实验Metabolic labeling Immunoprecipitation
pipet,then completely with narrow-mouth,gel-loading tip attached to P200 pipet so that you won’t take out ProteinG beads. 11.To the slightly moist pellet of beads,add 30µl SDS-PAGE sample buffer (either reducing or nonreducing,depending on the purpose
thoroughly with a narrow pipette. 2) Add 100 μl Dynabeads® to the prepared sample and vortex 2-3 seconds. 3) Incubate at 2 - 8°C for 30 min. with gentle tilting and rotation. 4) Fill the tube with cold Buffer
Metabolic labeling Immunoprecipitation
, then completely with narrow-mouth, gel-loading tip attached to P200 pipet so that you won’t take out ProteinG beads. 11. To the slightly moist pellet of beads,add 30µl SDS-PAGE sample buffer (either reducing or nonreducing, depending on the purpose) 12. Wait
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