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文献和实验Genome‐Wide Location Analysis by Pull Down of In Vivo Biotinylated Transcription Factors
system for titratable expression of transcription factors with C‐terminal FLAG and bio peptides. BirA recognizes and biotinylates the bio sequence on the central lysine residue (asterisk). (B ) Titration of tagged transcription factor expression by adjusting
., Ousterout, D.G., Brunger, J.M., Farin, A.M., Glass, K.A., Guilak, F., Crawford, G.E., Hartemink, A.J., and Gersbach, C.A. 2013. Synergistic and tunable human gene activation by combinations of synthetic transcription factors. Nat. Methods 10:239‐242
Chromatin Immunoprecipitation (ChIP)
, the ChIP method has been expanded to include ChIP-chip and ChIP-Seq assays. In ChIP studies designed to identify the locations of transcription factors and histones at a genome-wide scale (Ren et al. 2000; Weinmann et al. 2002; Hanlon and Lieb 2004
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