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文献和实验has made some useful shortcuts that work well if you are careful.Here is .This assay can be used to measure activity in small,snap frozen pieces of tissue (e.g.,colon polyps),or in cultured cells. 1.Homogenize Cells/Tissues For cells in culture: Harvest
Real Time PCR problem - different dilutions have the sam
(again) Setup your reaction using a different labs pipettes and workspace (different as in ANOTHER ROOM!) Oh, are you using TaqMan or SYBR Green? What C(T) are things coming up at? Also, is it for just one assay or all assays? -unknownphd- Hi
assayed at the same concentration, If in doubt about your samples or the quality of SAM, determine activity at two concentrations or do a concentration vs activity cuve prior to assaying all samples. 4. The Assay Proper Mix 15 m l lysate with 2 m l
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