thermo特约代理商|26612-500UL Pierce

蛋白标准品（Marker）知识汇总

的。 实验室常用的蛋白标准品多是来自NEB、MBI、GE、Bio-Rad 、Invitrogen、Novagen、Promega、MRESCO、Pierce Bio. 等品牌，生物帮2-D 蛋白 MW Markers产品推荐：Marker for 2D Electrophoresis 、ColorBurst(TM) Electrophoresis Marker 、Myoglobin, Carbamylated 2D Electrophoresis Marker from equine heart

Smolke:Protocols/Western

  Overview Blotting for large V5-tagged proteins in S. cerevisiae Materials Pierce ) Halt EDTA-free Protease Inhibitor (Pierce ) NuPAGE Novex Bis-Tris 4-12% mini gels (Invitrogen NP0321BOX) Prestained

纯化资料

+7 ml sample buffer 2. load 10 ml on gel 3. 10 ul prestained protein marker Dialysis: 1. transfer fractions containing proteins into membrane , tie each end, dialysis in 2 L PBS at 4°C o/n. Day 2. 1. Fresh PBS, dialysis another 2 h