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文献和实验Titration of Lentiviral Vectors
5-10%) Titer is a number (percentage) of cells transduced by a given vol and counted on D2 e.g. 1� gives you 10% of positivie cells and you had 50.000 cells on D2, so you have 5.000TU/� --> 5x106 TU/ml Day 7-8 isolate DNA for QPCR
慢病毒转染肝细胞方法Lentivirus Transduction of Hematopoietic Cells
Lentivirus Transduction of Hematopoietic Cells Ming-Jie Li and John J. Rossi Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
). Targeted HSC transduction is evaluated in the non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mouse model. RELATED INFORMATION An overview of approaches to modify lentivirus vectors for use in gene transfer
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