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上海玉博生物科技有限公司
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文献和实验M buffer - 1 hour. (Mix equal quantities of 2% aqueous OsO4 and 0.4M buffer and use immediately.) Rinse in 0.2M buffer - 5 mins. x 2. Dehydrate in 70% ethanol - 20 mins. x 2. (May be stored overnight at this stage if absolutely necessary
Preparing Colloidal Gold for Electron Microscopy
gold particles have become a useful tool for microscopists. Colloidal gold particles are especially useful for biological electron microscopy. Some of the reasons why are listed below. Homogeneous preparations of particles
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
-----------------------Gels:10.5 ml (20%/0.33%)acrylamide/bis acrylamide3.5 ml 10X TGOE buffer1.75 ml 50% glycerol35 microliters 0.5 M DTT20.9 ml H2O0.3 ml 10% ammonium persulfate30 microliters TEMED10X TG0E 500 ml0.25 M Tris 15.1 g Tris1.9 M glycine 71.3 g glycinepH
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