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Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides

both between species and for the same species when grown under different environmental conditions (1 –7 ,11 ). We are interested in the effects of rising atmospheric CO2 on plant gene expression. Exposure of plants to elevated CO2 (e.g., 100 ppm) often results

Plant Protein Isolation

C. Solutions and stuff A. Solutions 1. QB    Stock For 100ml  Final []  2M KPO4 (pH 7.8)  5ml  100mM  0.5M EDTA  200μL 1mM

FIXATION OF PLANT METAPHASE CHROMOSOMES

to weeks. (iv) a -bromonaphthalene: store water above liquid a -bromonaphthalene in a bottle (typically 100 ml over 50 ml). Shake, allow to separate, and then pipette out aliquots of the a -bromonaphthalene-saturated water-phase into small vials