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文献和实验PREPARATION OF 2% FORMALDEHYDE STOCK SOLUTION (2 METHODS)
METHOD 1: Formaldehyde preservative � 2% formaldehyde solution in protein-free phosphate-buffered saline (PBS). Prepare as follows: Add 2 g paraformaldehyde powder (e.g., Sigma, St. Louis, MO) to 100 ml
TISSUE PROCESSING AND PREPARATION FOR SECTIONING
It may be helpful to read the guidelines on the collection of pathological samples. Fixation: Specimens may be routinely fixed in 10% neutral buffered formalin (NBF). Fixation should be commenced as soon after surgical
处理光镜用组织 Processing tissues for light microscopy
Fixation: Specimens may be routinely fixed in 10% neutral buffered formalin (NBF). Fixation should be commenced as soon after surgical excision as is practically possible to prevent autolysis and bacterial decomposition.
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