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Analysis of TGF-Inducible Apoptosis

of specific heterodimeric cell-surface receptors (4 ). Although activation of TGF-β receptors initiates serine/threonine kinase activity, the subsequent signaling mechanisms involved in a wide array of diverse functional consequences

A SENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY

of actinomycin D (AD, C1 , e.g., Boehringer Mannheim) for biohazard considerations and/or to preserve samples for later analysis on the flow cytometer. For procedure see attached protocol. This staining procedure allows the correlation of apoptosis

Gene-Array Analysis of Glioma Cells After Treatment With an Anti-PKC siRNA: A General Protocol

interfering RNAs (siRNAs). Expression profiles were investigated on a human gene array of 588 key genes involved in DNA synthesis, apoptosis, cell-cell communication, intracellular signal-transduction pathways, cell-surface molecules and transcription factors