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上海玉博生物科技有限公司
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文献和实验Zymolyase Dissolve in 10% glucose. Store aliquots (25 µl) at 20o C in common freezer for stock solutions Use once and dispose Comments: alternative is to dissolve in SCE, 40% gycerol, 20 mg/ml final. Can use
Preparation of Microinjection Buffer
, it is suggested that small quantities (1 gram) should be ordered and then all of it should be prepared at once. Microinjection Buffer For 50 ml: 10 mM Tris-HCl, pH 7.5 0.5 ml of 1 M Tris-HCl, pH 7.5 (autoclaved) 0.1 mM EDTA, pH 8.0 10 microliters of 0.5 M
Mag-Bind Blood DNA Maximum Yield Protocol (2ml-6ml)
. Add 10ml SPM Buffer to the sample. 14. Resuspend the Mag-Bind particles pellet by vortexing. Incubate 3 minutes at room temperature. Complete resuspension of the Mag- Bind particles pellet is critical to obtain good results. 15. Place
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