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文献和实验Limited, Nepean, ON, Canada; Catalog No. 03-337-15) or 1L glass bottle. 3. 40% formaldehyde solution (prepared on the day of fixing from paraformaldehyde): In scintillation vial, mix 0.92 g paraformaldehyde in 2.5 mL water containing 35 m L of 1N
less than ~200 µl worms here, if you have more then split into two tubes. Should get 200 µl worms from 1-3 large plates). Move to a 1.5 ml eppendorf tube. At this point, can throw the worms into the -20° freezer to synchronize preps of different strains. Freezing
and pellet the worms in 4 ml TEN. Remove the supernatant, resuspend to a total volume of .5 ml (note: use less than ~200 µl worms here, if you have more then split into two tubes. Should get 200 µl worms from 1-3 large plates). Move to a 1.5 ml eppendorf tube
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