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- 2025年07月15日
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上海玉博生物科技有限公司
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文献和实验in the past - Sigma #N-4388; so we now use powdered media from Gibco #21700-075 or liquid media from Gibco #11765-054. Neither of these have Hepes buffering) 1) Start by rinsing a graduated cylinder three times ddH2 O. 2) Then fill the cylinder to 600-ml
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
DMEM/F-12. 3) Add the diluted primary antibody to the cells (6 mL per 100-mm dish). 4) Incubate the primary antibody and the cells at 37°C for 60 minutes. 5) Remove the primary antibody solution from the dish. Note: The primary
Mixing F-12 (HAM) Media 1) Start by rinsing a graduated cylinder three times ddH2 O. 2) Then fill the cylinder to 600-ml with ddH2 O. 3) Add one bottle of F-12 (HAMS) (Sigma #N-4388). 4) Stir this until all of the powder
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