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上海玉博生物科技有限公司
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文献和实验). 3. Tris-buffered saline (TBS) with Tween (TBS-T): Prepare10× stock with 1.37 M NaCl, 27 mM KCl, 250 mM Tris-HCl, pH 7.4, and 1% (v/v) Tween-20. 4. Blocking buffer: 4% (w/v) bovine serum albumin (BSA) and0.1% (w/v) sodium azide in TBS. 5. Primary
Alkaline Southern Blotting Procedure
and an additional 1 sec on gel alone. Invert gel, place into .25M HCl for 7 min. Rinse briefly in dH2O. Soak in 0.3M NaOH,0.3M NaCl for 10 min. Set up a transfer tray (9 X 12 inch glass baking dish) by pouring transfer solution in the tray (about 1000 ml
, cat. no. 1.07687) Imidazole (AppliChem, cat. no. A10730) Bovine serum albumin, BSA (AppliChem, cat. no. A1391) D -Glucose (Merck, cat. no. 1.08342) Ethanol (Merck, cat. no. 1.00983) Sodium hydroxide
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