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上海玉博生物科技有限公司
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文献和实验,safety glasses,and gloves.Care should be taken to avoid contact with skin or eyes.This protocol requires the use of ethanol and formamide.Gel reagents may contain acrylamide,a neurotoxin and suspected carcinogen.Please follow the manufacturer’s Material
Membrane Transfer and Crosslinking for RNA
that disadvantage. For this reason, we recommend using Ambion's BrightStar™-Plus membranes (Cat #10100), which are positively charged nylon and have a high affinity for nucleic acids. Optimal Transfer Conditions Denaturing Agarose
cliPS . 10% Denaturing Acrylamide gel, 100ml (for 20x40 cm glass plates 1.5mm thick) Swirl and warm up to 42C to allow for rapid polymerization (Heating is optional and not suggested for first-timers ) Pour gel: Add 678 μl
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