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文献和实验Electroporation of Helicobacter pylori
The genus Helicobacter currently comprises over 15 different species with members described as microaerophilic gram-negative spiral rods with a G + C content of 35–38%. The most medically important Helicobacter is Helicobacter pylori
The Helicobacter pylori cag Pathogenicity Island
The cag pathogenicity island is a well-characterized virulence determinant. It is composed of 32 genes that encode a type IV bacterial secretion system and is linked with a more severe clinical outcome. The following chapters will explore
Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice
labeled polyclonal antisera or in situ hybridization for H. pylori rRNA. In this chapter, we will summarize strategies to allow initiation of experiments with helicobacter-free mice and then focus on PCR and ELISA techniques to verify and quantify H
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