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文献和实验A recent development is the fluorescence-based thermal shift (ThermoFluor) assay. It uses an environmentally sensitive dye, Sypro Orange, to monitor the thermal stability of a protein and investigate factors (e.g., buffers, additives, and ligands) affecting
Introduction to the EMSA (Gel Shift) Technique
and determining protein:DNA interactions is the electrophoretic mobility shift assay (EMSA). The EMSA technique is based on the observation that protein:DNA complexes migrate more slowly than free DNA molecules when subjected to non-denaturing polyacrylamide
separation between incorporated and unincorporated dye should be visible. The collected blue-coloured fraction of fluorescently labeled antigen can be used in band-shift assays. Native gel electrophoresis Protein samples (4 µl) are diluted
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