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文献和实验Isolation of micro-RNA (miRNA)
Adapted from the Instruction manual of Stratagene''s Micro RNA Isolation Kit (Catalog #200344) Overview There are two main methods for isolating RNA: phenol-based extraction and silica matrix or glass fiber filter (GFF
of 260/280 of 1.8-2.0 indicates purity 1. Dilute 1-10 %26micro;l of DNA into 500 %26micro;l H2O. 2. Place in a quartz cuvette and measure the OD260 and OD280. 3. Calculate the DNA concentration using the following equation:
of the unknown samples. PROCEDURE (MICRO ASSAY, 1-10 µ g protein; Prepare standard concentrations of BSA of 1, 5, 7.5 and 10 µ g/ml. Prepare a blank of NaCl only. Prepare a series of sample dilutions. Add 100 µ l
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