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文献和实验Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
) could be detected in the bound fraction after precipitation with conventional mono- and polyclonal antibodies (Figure 1) (Rothbauer et al., 2008). The lack of unspecific binding or antibody fragments is one major advantage of the GFP-Trap, because unspecific protein
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
7.4 buffer used here. However, some tissues also have a cytosolic form of ß-gal which can show enough activity to be confounding. The variables that affect background staining include the fixative type, length of time in fixative, pH of the buffer
【共享】流式 Overview of Flow Cytometry
). For polyclonal reagents, 40 to 100 µg/ml are generally required to reach saturation.Once the saturating concentration is determined, the total amount of antibody is calculated for staining the desired cell number in bulk by assuming the cells are being stained
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