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文献和实验.1 RNA Probe Preparation (see Note 1 ) 1. 1.5 mL microcentrifuge tubes or standard 96-well V-bottom microplates. 2. RNAse free water. 3. T7, T3 or SP6 RNA Polymerase (Fermentas Life Sciences, Burlington, ON, Canada
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
I that is stored at ?80°C (see Note 2). 2. Silicon oil. 3. QIAquick Nucleotide Purification Kit (Qiagen). 4. QIAquick PCR Purification Kit (Qiagen). 5. Splinkerette oligonucleotides (see Table 1 for sequences). Resuspended in TE buffer
processing and avoid manual manipulations that introduce bias, we coded our own filtering and data analysis software in the JAVA Programming Language using the Java Development Kit and Advanced Imaging Library from Sun Microsystems.III. Procedures
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