clontech特约代理商|638314 Mir-X™ mi

Isolate It All：siRNA • miRNA • Total RNA • Native Protein

or by solution hybridization assay with the mir Vana™ miRNA Detection Kit (Ambion). RNA probes were prepared by in vitro transcription (mRNA probes--MAXIscript® Kit, Ambion) or by 5' end labeling (rRNA, miRNA and siRNA probes - mir Vana™ Probe & Marker Kit

Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I

RNA using the “PolyAtract® mRNA Isolation System III” kit (Promega, USA). Messenger RNA was reverse transcribed with oligo (dT) 15 using “Reverse Transcription System” (Promega). Polymerase chain reactions were set up in 50-μl volumes using “PCR Master