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文献和实验Differentiation of Human Embryonic Stem Cells to Cardiomyocytes on Microcarrier Cultures
for human embryonic stem cell differentiation to cardiomyocytes in suspended microcarrier cultures. Tissue Eng. C 16:1609‐1619. Lock, L.T. and Tzanakakis, E.S. 2009. Expansion
Establishment of Spontaneously Immortalized Keratinocyte Lines from Wild-Type and Mutant Mice
and sometimes not sufficient for certain biochemical analyses. To overcome this restriction some permanent murine keratinocyte lines have been generated by transfection with SV40T or HPV E6E7 genes. This is, however, not suitable if established or hypothetical biochemical
Immortalization of Human Articular Chondrocytes for Generation of Stable, Differentiated Cell Lines
Immortalized chondrocytes of human origin have been developed to serve as reproducible models for studying chondrocyte function. In this chapter, methods for immortalization of primary human chondrocytes with SV40-TAg, HPV-16 E6/E7
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