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上海玉博生物科技有限公司
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文献和实验Cyanine Dye Purification Protocol
Slowly add 111.3 mL (80.95 g) HPLC grade triethylamine; the reaction is exothermic; the solution will become cloudy � wait until it clears before adding more) Add dropwise an additional 10-15 mL acetic acid to adjust the pH to 8
Tetra‐n ‐butylammonium fluoride (TBAF; Aldrich) Tetrahydrofuran (THF; Acros) HPLC buffer A: 0.1 M triethylammonium acetate
1 ) Dry acetonitrile (MeCN), HPLC grade (store over 4‐Å molecular sieves) 2′‐Deoxyribonucleoside phosphoramidites (Glen Research
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