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上海玉博生物科技有限公司
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文献和实验Semi-Quantitative Measurement of Proteins by Dot Blotting
mM Tris-HCl, 150mM NaCl, pH 7.5 TBS-T: 0.05% Tween 20 in TBS BSA/TBS-T: 0.1% BSA in TBS-T Nitrocellulose membrane (BIO-RAD, Trans-Blot™ etc) Procedure 1. Have nitrocellulose membrane (NOT PVDF!) ready, draw grid by pencil to indicate
Semi-Quantitative Measurement of Proteins by Dot Blotting
mM Tris-HCl, 150mM NaCl, pH 7.5 TBS-T: 0.05% Tween 20 in TBS BSA/TBS-T: 0.1% BSA in TBS-T Nitrocellulose membrane (BIO-RAD, Trans-Blot™ etc) Procedure 1. Have nitrocellulose membrane (NOT PVDF!) ready, draw grid by pencil to indicate
Microarray Analysis of DNA Replication Timing
Cell culture: Dulbecco’s Modified Eagle’s Medium (DMEM; CELLGRO) supplemented with 10% iron supplemented donor calf serum (CELLECT) and 1% Penicillin-Streptomycin (GIBCO). 2. Synchronization: 1
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