NeuroMab特约代理商|75-124 Anti-GABA

Protocol for anti-HA antibody Western Blotting

of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody

Characterization of GABA Receptors

, or ICN Biomedicals) [3 H]γ‐Amino‐n ‐butyric acid (GABA; 25 to 40 Ci/mmol; PerkinElmer NEN) (±)‐Baclofen or GABA (unlabeled

Protocol for anti-HA antibody Western Blotting

of interest. 2) Transfer proteins in acrylamide gel to nylon membrane 3) Block overnight @4C in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 (important if you are re-using