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上海玉博生物科技有限公司
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文献和实验, Inc. ), the latter being less hazardous than the first. 1st injection: 15 - 50 ug Antigen in 200 ul adjuvant, injected subcutaneously. 2nd injection: 2 weeks later; boost with 15 - 50 ug of Antigen in adjuvant. (If time permits, boost again a month later). 7 - 10 days
Polyclonal and Monoclonal Antibodies
% ( 1000X ) Protocol: 1) ADSORBTION OF ANTIGEN a) Dilute Ag to 10 ug/ml in PBS. b) Add 100 ul of Ag solution to each well. c) Leave O.N covered with saran wrap, at 4 C. 2) BLOCKING a) Wash unbound Ag by inverting
monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
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