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上海玉博生物科技有限公司
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文献和实验4× Hybridization buffer (200 mM HEPES, pH 7.5/2 M NaCl) Lyophilized DSN (see recipe for preparation of 1U/µl DSN solution
Construction of Gene-Targeting Vectors by Recombineering
/mL of Bsd or 1-5 µg/mL of Puro. HPLC (high performance liquid chromatography)-purified primers (Sigma-Genosys) for isolation of selection cassettes (see Step 2) HPLC-purified long oligos perform better than desalted-only oligos. HPLC
. Chem. 389:1017‐1031. Beausoleil, S.A., Villen, J., Gerber, S.A., Rush, J., and Gygi, S.P. 2006. A probability‐based approach for high‐throughput protein phosphorylation
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