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文献和实验ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer Day -2: o Start a 5ml overnight culture from a single colony. Day -1: o Start a larger overnight culture using part of the 5ml culture (subculture). Day
2A ), ice cold FA lysis buffer with and without 2 mM PMSF (see recipe ), ice cold ChIP elution buffer (see recipe
Propagation, Quantification, Detection, and Storage of West Nile Virus
) 25% glycerol in TNE (v/v) (see recipe for TNE) TNE (see recipe ) T
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