immunostep特约代理商|FCR-2ML FcR bl

Blocking of unwanted non-specific staining

and other cell types. By exploiting the preferential avidity of Fc receptor for human > mouse Ig> rabbit > swine > goat immunoglubulins, one may use a blocking of the receptors with a reagent which will not interfere with the secondary reagents

Western blotting using ECL reagent

membrane (Amersham Life Science; cat # RPN2020D)Kodak X-OMAT (XAR-5, 18X24 cm; cat # 8532665)10X TBS12.11 g Tris-base (100 mM)87.66 g NaCl (1500 mM)1 liter dd H2OAdjust pH= 7.6Washing buffer (TBS-T): 100 ml 10X TBS + 900 ml dd H2O + 1 ml Tween-20.Blocking

Blocking cDNA arrays printed on amine slides

so they can hybridize to the fluorescently labeled probe DNA . During the blocking step the array will disappear so be sure that the boundary of the array has been marked with a diamond pin as described in microarray construction. During the process, the exposed amine