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文献和实验【共享】经验:The RNeasy Mini Kit from Qiagen
The RNeasy Mini Kit from Qiagen is designed for RNA extraction, although the basic format is similar to other Qiagen products designed to purify nucleic acids: ion exchange columns specifically bind RNA which is purified by wash steps
Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
and thereby ensure maximal growth and plasmid DNA yields). 2nd Day -DNA extraction and purification Materials required QIAGEN Mini-prep kit Microfuge Cell Lysis/DNA Extraction 1. Concentrate the bacteria by transferring 1 ml bacterial suspension
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