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- 2025年07月15日
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上海玉博生物科技有限公司
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文献和实验ES Cell Culture and Manipulation
tips (preferably barrier, or plugged tips), a sterile 96well round-bottom plate, plenty of ES cell media, and an inverted microscope in a hood. Aspirate the REF media from the 24-wells and add 2ml of ES cell media to each well. Add 40 µl trypsin-EDTA
ES Cell Culture and Manipulation
), a sterile 96well round-bottom plate, plenty of ES cell media, and an inverted microscope in a hood. Aspirate the REF media from the 24-wells and add 2ml of ES cell media to each well. Add 40 µl trypsin-EDTA to as many wells of the 96-well plate as you plan
, 1.5 mL and 0.5 mL microcentrifuge tubes, or 0.2 mL half-skirted 96-well PCR plates (Abgene, Rochester, NY, USA; Catalog No. AB-0900). 2. Microplate sealing foil (Ultident, Saint-Laurent, QC, Canada; Catalog No. 24-PCR-AS-200). 3. PBT
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